Institute of Molecular Systems Biology

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Publications

Here is a full list of all publications from the group since 2020, with links to the publication. For a complete overview, see Alexander's author profiles on external page ORCID, external page Scopus or external page Web of Science.

(Lab members are highlighted in bold, * means equal contribution)

  • Cai B., Xu J., Collet E. H., Aarts E., Luo L., Leitner A., Ishikawa T., Beltrao P., Pearson C. G., Pilhofer M., Wieczorek M., Structure and assembly of the A-C linker connecting microtubule triplets in centrioles. Sci. Adv. 11 (2025) eady3689. external page Link to paper
  • Rappsilber J., Bruce J., Combe C., Fried S. D., Graziadei A., Heck A. J. R., Iacobucci C., Leitner A., Mechtler K., Novak P., O’Reilly F., Schriemer D. C., Sinz A., Stengel F., Thalassinos K., A Roadmap for Improving Reliability and Data Sharing in Crosslinking Mass Spectrometry. Mol. Cell. Proteomics 24 (2025) 101024. external page Link to paper 
  • Loeff L., Kroupova A., Asanovic I., Boneberg F. M., Pfleiderer M. M., Riermeier L., Leitner A., Ferdigg A., Ackle F., Martinez J., Jinek M., Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation. Nat. Struct. Mol. Biol. 32 (2025) 1205-1212. external page Link to paper
  • Poovathumkadavil D.*, Reynolds-Winczura A.*, Skagia A., Passaretti P., Sadurni M. M., Kingsley G., Leitner A., Saponaro M., Gambus A., CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS. Nucl. Acids Res. 53 (2025) gkaf530. external page Link to paper
  • de Ceuninck van Capelle C., Luo L., Leitner A., Tschanz S. A., Latzin P., Ott S., Herren T., Müller L., Ishikawa T., Proteomic and structural comparison between cilia from primary ciliary dyskinesia patients with a DNAH5 defect. Front. Mol. Biosci. 12  (2025) 1593810. external page Link to paper
  • Sarnowski C. P., Knörlein A., de Vries T., Götze M., Beusch I., Aebersold R., Allain F. H.-T., Hall J., Leitner A., A highly sensitive protein-RNA cross-linking mass spectrometry workflow with enhanced structural modeling potential. Nucl. Acids Res. 53 (2025) gkaf523. external page Link to paper
  • Trabjerg E., Nilsen J., Gjølberg T. T., Andersen J. T., Leitner A., Rand K. D., Identification of a direct interaction between the Fab domains of IgG antibodies and human FcRn upon IgG-FcRn complex formation. Commun. Biol. 8 (2025) 922. external page Link to paper
  • Martin F., Kazrani A. A., Lafouge J., Diaz-Jimenez D. M., Siebert S., Fabbro-Burtschell L., Maillard E., Lapouge K., Mertens H. D. T., Sauter C., Leitner A., Ochsenbein F., Blais A., Bergamin E., Structure of the nucleosome-bound human BCL7A. Nucl. Acids Res. 53 (2025) gkaf273. external page Link to paper
  • von Rosen T., Zdanowicz R., El Hadeg Y., Afanasyev P., Boehringer D., Leitner A., Glockshuber R., Weber-Ban E., Substrates bind to residues lining the ring of asymmetrically engaged bacterial proteasome activator Bpa. Nat. Commun. 16 (2025) 3042. external page Link to paper
  • Usher S., Toulmé E., Florea R., Yatskevich S., Jao C. C., Dijkhof L. R. H., Colding J. M., Joshi P., Zilberleyb I., Trimbuch T., Brokowski B., Hauser A. S., Leitner A., Rosenmund C., Kschonsak M., Pless S. A., The sodium leak channel NALCN is regulated by neuronal SNARE complex proteins. Sci. Adv. 11 (2025) eads6004. external page Link to paper
  • Mohammadi A., Deroo S., Leitner A., Stengel F., Krammer E.-M., Aebersold R., Prévost M., Raussens V., Characterization of the N- and C-terminal domain interface of the three main apoE isoforms: a combined quantitative cross-linking mass spectrometry and molecular modelling study. Biochim. Biophys. Acta – Gen. Subj. 1869 (2025) 130768. external page Link to paper
  • Portugal-Calisto D., Geiger A. G., Rabl J., Vadas O., Oborská-Oplová M., Mazur J., Richina F., Klingauf-Nerurkar P., Michel E., Leitner A., Boehringer D., Panse V. G., An inhibitory segment within G-patch activators tunes Prp43-ATPase activity during ribosome assembly. Nat. Commun. 15 (2024) 10150. external page Link to paper
  • Álvarez L.*, Haubrich K.*, Iselin L., Gillioz L., Ruscica V., Lapouge K., Augsten S., Huppertz I., Choudhury N. R., Simon B., Masiewicz P., Lethier M., Cusack S., Rittinger K., Gabel F., Leitner A., Michlewski G., Hentze M. W., Allain F. H. T., Castello A., Hennig J., The molecular dissection of TRIM25’s RNA-binding mechanism provides key insights into its antiviral activity. Nat. Commun. 15 (2024) 8485. external page Link to paper
  • Zdanowicz R., Afanasyev P., Pruška A., Harrison J. A., Giese C., Boehringer D., Leitner A., Zenobi R., Glockshuber R., Stoichiometry and architecture of the human pyruvate dehydrogenase complex. Sci. Adv. 10 (2024) eadn4582. external page Link to paper
  • Combe C. W., Kolbowski L., Fischer L., Koskinen V., Klein J., Leitner A., Jones A. R., Vizcaíno J. A., Rappsilber J., mzIdentML 1.3.0 – Essential progress on the support of crosslinking and other identifications based on multiple spectra. Proteomics 24 (2024) 2300385. external page Link to paper
  • Vallat B., Webb B. M., Westbrook J. D., Goddard T. D., Hanke C. A., Graziadei A., Peisach E., Zalevsky A., Sagendorf J., Tangmunarunkit H., Voinea S., Sekharan M., Yu J., Bonvin A. A. M. J. J., DiMaio F., Hummer G., Meiler J., Tajkhorshid E., Ferrin T. E., Lawson C. L., Leitner A., Rappsilber J., Seidel C. A. M., Jeffries C. M., Burley S. K., Hoch J. C., Kurisu K., Morris K., Patwardhan A., Velankar S., Schwede T., Trewhella J., Kesselman C., Berman H. M., Sali A,. IHMCIF: An Extension of the PDBx/mmCIF Data Standard for Integrative Structure Determination Methods. J. Mol. Biol. 436 (2024) 168546. external page Link to paper
  • Khanppnavar B.*, Schuster D.*, Lavriha P., Uliana F., Özel F., Mehta V., Leitner A., Picotti P., Korkhov V. M., Regulatory sites of CaM-sensitive adenylyl cyclase AC8 revealed by cryo-EM and structural proteomics. EMBO Rep. 25 (2024) 1513-1540. external page Link to paper
  • de Vries T.*, Novakovic M.*, Ni Y., Smok I., Inghelram C., Bikaki M., Sarnowski C. P., Han Y., Emmanouilidis L., Padroni G., Leitner A., Allain F. H.-T., Specific protein-RNA interactions are mostly preserved in biomolecular condensates. Sci. Adv. 10 (2024) eadm7435. external page Link to paper
  • Park J.*, Kim H.*, Gestaut D.*, Lim S., Opoku-Nsiah K., Leitner A., Frydman J., Roh S.-H., A structural vista of phosducin-like PhLP2A-chaperonin TRiC cooperation during the ATP-driven folding cycle. Nat. Commun. 15 (2024) 1007. external page Link to paper
  • Begley A., Oganesyan I., Mrđenović D., Smok I., Leitner A., Zenobi R., Low-temperature plasma jet treatment generates reactive oxygen species in solution that leads to peptide oxidation and protein aggregation. J. Chem. Phys. D 57 (2024) 475206. external page Link to paper
  • Selcuk K., Leitner A., Braun L., Le Blanc F., Pacak P., Pot S., Vogel V., Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers. Matrix Biol. 125 (2024) 113-132. external page Link to paper
  • Zila N., Eichhoff O. M., Steiner I., Mohr T., Bileck A., Cheng P. F., Leitner A., Gillet L., Sajic T., Goetze S., Friedrich B., Bortel P., Strobl J., Reitermaier R., Hogan S. A., Martínez-Goméz J. M., Staeger R., Tuchmann F., Peters S., Stary G., Kuttke M., Elbe-Buerger A., Hoeller C., Kunstfeld R., Weninger W., Wollscheid B., Dummer R., French L. E., Gerner C., Aebersold R., Levesque M. P., Paulitschke V., Proteomic profiling of advanced melanoma patients to predict therapeutic response to anti-PD-1 therapy. Clin. Cancer Res. 30 (2024) 159-175. external page Link to paper
  • Kern C., Radon C., Wende W., Leitner A., Sträßer K., Cross-linking mass spectrometric analysis of the endogenous TREX complex from S. cerevisiae. RNA 29 (2023) 1870-1880. external page Link to paper
  • Hau J.-L., Kaltwasser S., Muras V., Casutt M. S., Vohl G., Claußen B., Steffen W., Leitner A., Bill E., Cutsail III G. E., DeBeer S., Vonck J., Steuber J., Fritz G., Conformational coupling of redox-driven Na+-translocation in Vibrio cholerae NADH:quinone oxidoreductase. Nat. Struct. Mol. Biol. 30 (2023) 1686-1694. external page Link to paper
  • Dorn G.*, Gmeiner C.*, de Vries T.*, Dedic E., Novakovic M., Damberger F. F., Maris C., Finol E., Sarnowski C. P., Kohlbrecher J., Welsh T. J., Bolisetty S., Mezzenga R., Aebersold R., Leitner A., Yulikov M., Jeschke G., Allain F. H.-T., Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility. Nat. Commun. 14 (2023) 6429. external page Link to paper
  • Sarnowski C. P., Götze M., Leitner A., RNxQuest: An Extension to the xQuest Pipeline Enabling Analysis of Protein–RNA Cross-Linking/Mass Spectrometry Data. J. Proteome Res. 22 (2023) 3368-3382. external page Link to paper
  • Betancourt Moreira K.*, Collier M. P.*, Leitner A., Li K. H., Serrano Lachapel I. L., McCarthy F., Opoku-Nsiah K. A., Morales-Polanco F., Barbosa N., Gestaut D., Samant R. S., Roh S.-h., Frydman J., A hierarchical assembly pathway directs the unique subunit arrangement of TRiC/CCT. Mol. Cell. 83 (2023) 3123-3139. external page Link to paper
  • Padroni G.*, Bikaki M.*, Novakovic M.*, Wolter A. C., Rüdisser S. H., Gossert A. D., Leitner A., Allain F. H.-T., A hybrid structure determination approach to investigate the druggability of the nucleocapsid protein of SARS-CoV-2. Nucl. Acids Res. 51 (2023) 4555-4571. external page Link to paper
  • Eichhoff O., Stoffel C. I., Käsler J., Briker L., Turko P., Karsai G., Zila N., Paulitschke V., Cheng P. F., Leitner A., Bileck A., Zamboni N., Irmisch A., Balazs Z., Tastanova A., Pascoal S., Johansen P., Wegmann R., Mena J., Othman A., Viswanathan V. S., Wenzina J., Aloia A., Saltari A., Dzung A., TuPro Consortium, Krauthammer M., Schreiber S. L., Hornemann T., Distel M., Snijder B., Dummer R., Levesque M. P., ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma, Cancer Res. 83 (2023) 1128-1146. external page Link to paper
  • Barret D. C. A.*, Schuster D.*, Rodrigues M. J., Leitner A., Picotti P., Schertler G. F. X., Kaupp U. B., Korkhov V. M., Marino J., Structural basis of calmodulin modulation of the rod cyclic nucleotide-gated channel, Proc. Natl. Acad. Sci. USA 120 (15) e2300309120. external page Link to paper
  • Steinmetz B.*, Smok I.*, Bikaki M., Leitner A., Protein–RNA interactions: from mass spectrometry to drug discovery (review article), Essays Biochem. 67 (2023) 175-186. external page Link to paper
  • Pavic K., Gupta N., Domènech Omella J., Derua R., Aakula A., Huhtaniemi R., Määttä J. A., Höfflin N., Okkeri J., Wang Z., Kauko O., Varjus R., Honkanen H., Abankwa D., Köhn M., Hytönen V. P., Xu W., Nilsson J., Page R., Janssens V., Leitner A., Westermarck J., Structural mechanism for inhibition of PP2A-B56α and oncogenicity by CIP2A, Nat. Commun. 14 (2023) 1143. external page Link to paper
  • Burke D. F.*, Bryant P.*, Barrio-Hernandez I.*, Memon D.*, Pozzati G.*, Shenoy A., Zhu W., Dunham A. S., Albanese P., Keller A., Scheltema R. A., Bruce J. E., Leitner A., Kundrotas P., Beltrao P., Elofsson A., Towards a structurally resolved human protein interaction network, Nat. Struct. Mol. Biol. 30 (2023) 216-225. external page Link to paper
  • Gestaut D.*, Zhao Y.*, Park J., Ma B., Leitner A., Collier M., Pintilie G., Roh S.-H., Chiu W., Frydman J., Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT, Cell 185 (2022) 4770-4787. external page Link to paper
  • Knörlein A., Sarnowski C. P., de Vries T., Stoltz M., Götze M., Aebersold R., Allain F. H.-T., Leitner A., Hall J., Nucleotide-amino acid π-stacking interactions initiate photo cross-linking in RNA-protein complexes, Nat. Commun. 13 (2022) 2719. external page Link to paper
  • Gorbovytska V.*, Kim S.-K.*, Kuybu F., Götze M., Um D., Kang K., Pittroff A., Brennecke T., Schneider L.-M., Leitner A., Kim T.-K., Kuhn C.-D., Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF, Nat. Commun. 13 (2022) 2429. external page Link to paper
  • Sarnowski C. P., Bikaki M., Leitner A., Cross-linking and mass spectrometry as a tool for studying the structural biology of ribonucleoproteins (review article), Structure 30 (2022) 441-461. external page Link to paper
  • Trabjerg E., Keller A., Leitner A., pH Dependence of Succinimide-Ester-Based Protein Cross-Linking for Structural Mass Spectrometry Applications, ACS Meas. Sci. Au 2 (2022) 132-138. external page Link to paper
  • Kschonsak M., Chua H. C., Weidling C.*, Chakouri N.*, Noland C. L.*, Schott K., Chang T., Tam C., Patel N., Arthur C. P., Leitner A., Ben-Johny M., Ciferri C., Pless S. A., Payandeh J., Structural architecture of the human NALCN channelosome, Nature 603 (2022) 180-186. external page Link to paper
  • de Vries T.*, Martelly W.*, Campagne S.*, Sabath K., Sarnowski C. P., Wong J., Leitner A., Jonas S., Sharma S., Allain F. H.-T., Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly, Proc. Natl. Acad. Sci. USA 119 (2022) e2114092119. external page Link to paper
  • Leitner A., Chemical derivatization of peptides for quantitative proteomics (book chapter), in: Advances in Chemical Proteomics (Ed., X. Yao), Elsevier, 2022, pp. 75-94. external page Link to paper
  • Kroupova A., Ackle F., Asanović I., Weitzer S., Boneberg F. M., Faini M., Leitner A., Chui A., Aebersold R., Martinez J., Jinek M., Molecular architecture of the human tRNA ligase complex, eLife 10 (2021) e71656. external page Link to paper
  • Götze M., Sarnowski C. P., de Vries T., Knörlein A., Allain F. H.-T., Hall J., Aebersold R., Leitner A., Single Nucleotide Resolution RNA–Protein Cross-Linking Mass Spectrometry: A Simple Extension of the CLIR-MS Workflow, Anal. Chem. 93 (2021) 14626-14634. external page Link to paper
  • Mohamed W. I., Park S. L.*, Rabl J.*, Leitner A., Boehringer D., Peter M., The human GID complex engages two independent modules for substrate recruitment, EMBO Rep. 22 (2021) e52981. external page Link to paper
  • Gerhardy S.*, Oborská-Oplová M.*, Gillet L., Börner R., van Nues R., Leitner A., Michel E., Petkowski J., Grannemann S., Sigel R., Aebersold R., Panse V. G., Puf6 primes 60S pre-ribosome nuclear export at low temperature, Nat. Commun. 12 (2021) 4696. external page Link to paper
  • Collier M. P., Betancourt Moreira K., Li K. H., Chen Y.-C., Itzhak D., Samant R., Leitner A., Burlingame A., Frydman J., Native mass spectrometry analyses of chaperonin complex TRiC/CCT reveal subunit N-terminal processing and re-association patterns, Sci. Rep. 11 (2021) 13084. external page Link to paper
  • Knowlton J. J.*, Gestaut D.*, Ma B., Taylor G., Seven A. B., Leitner A., Wilson G. J., Shanker S., Yates N. A., Prasad B. V. V., Aebersold R., Chiu W., Frydman J., Dermody T. S., Structural and functional dissection of reovirus capsid folding and assembly by the prefoldin-TRiC/CCT chaperone network, Proc. Natl. Acad. Sci. USA 118 (2021) e2018127118. external page Link to paper
  • Mohammadi A., Tschanz A., Leitner A., Expanding the Cross-Link Coverage of a Carboxyl-Group Specific Chemical Cross-Linking Strategy for Structural Proteomics Applications. Anal. Chem. 93 (2021) 1944-1950. external page Link to paper
  • Leitner A., Bonvin A. M. J. J., Borchers C. H., Chalkley R. J., Chamot-Rooke J., Combe C. W., Cox J., Dong M.-Q., Fischer L., Götze M., Gozzo F. C., Heck A. J. R., Hoopmann M. R., Huang L., Ishihama Y., Jones A. R., Kalisman N., Kohlbacher O., Mechtler K., Moritz R. L., Netz E., Novak P., Petrotchenko E., Sali A., Scheltema R. A., Schmidt C., Schriemer D., Sinz A., Sobott F., Stengel A., Thalassinos K., Urlaub H., Viner R., Vizcaino J. A., Wilkins M. R., Rappsilber J., Toward Increased Reliability, Transparency, and Accessibility in Cross-linking Mass Spectrometry, Structure 28 (2020) 1259-1268. external page Link to paper
  • Jung T.*, Shin B.*, Tamo G.*, Kim H.*, Vijayvargia R.*, Leitner A., Marcaida M. J., Astorga-Wells J., Jung R., Aebersold R., Del Peraro M., Hebert H., Seong I. S., Song, J.-J., The Polyglutamine Expansion at the N-Terminal of Huntingtin Protein Modulates the Dynamic Configuration and Phosphorylation of the C-Terminal HEAT Domain. Structure 28 (2020) 1035-1050. external page Link to paper
  • Poghosyan E., Iacovache I., Faltova L., Leitner A., Yang P., Diener D. R., Aebersold R., Zuber B., Ishikawa T., The structure and symmetry of radial spoke protein complex in Chlamydomonas flagella. J. Cell Sci. 133 (2020) jcs245233. external page Link to paper
  • Arora Verasztó H., Logotheti M., Albrecht R., Leitner A., Zhu H., Hartmann M. D., Architecture and functional dynamics of the pentafunctional AROM complex. Nat. Chem. Biol. 16 (2020) 973-978. external page Link to paper
  • Sabath K., Stäubli M. L., Marti S., Leitner A., Moes M., Jonas S., INTS10–INTS13–INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module, Nat. Commun. 11 (2020) 3422. external page Link to paper
  • Klatt F., Leitner A., Kim I. V., Ho-Xuan H., Schneider E. V., Langhammer F., Weinmann R., Müller M. R., Huber R., Meister G., Kuhn C.-D., A precisely positioned MED12 activation helix stimulates CDK8 kinase activity, Proc. Natl. Acad. Sci. USA 117 (2020) 2894-2905. external page Link to paper
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