Novel metabolomics method for α-keto acids

Alpha-Keto acids (e.g. glyoxylate, oxaloacetate, pyruvate, ...) are highly reactive and thus unstable compounds. We established a new method for in situ stabilization and quantification by mass spectrometry. The technique also allows determination of labeling patterns.

Reference

Zimmermann M, Sauer U, Zamboni N, Quantification and Mass Isotopomer Profiling of α-Keto Acids in Central Carbon Metabolism, Analytical Chemistry, 2014, 86 (6), pp 3232–3237 external page DOI

JavaScript has been disabled in your browser