LiP-SRM paper highlighted in Nature Methods
Our limited proteolysis SRM paper was highlighted in Nature Methods.
by
Paul Boersema
Probing the protein structure-ome
Protein structure changes can be charted on a global scale with a method that couples limited proteolysis with mass spectrometry–based proteomics.
The interior of the cell is like a city sidewalk at rush hour, crowded with dynamic entities bumping elbows and occasionally stopping to interact. A perturbation, such as an encounter with a ligand or a change in the environment, can cause structural changes to proteins. Although a number of methods exist to probe protein structural changes on the individual level, broader approaches that allow researchers to follow changes on a global scale upon perturbation have been lacking.