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The Sauer Lab developed an experimental-computational approach to identify regulatory needles in metabolic haystacks. By using steady-state ¹³C flux, metabolites and transcript data, the approach revealed the sparse and transition-dependent regulators that drive transitions between carbon sources in Escherichia coli. Thus, pseudo-transition analysis is a novel approach to efficiently explore the vast regulatory landscape of dynamic adaptations using relatively few stationary observations.

The research of our lab focuses on cell surface protein in general and specifically on receptors. We recently suceeded in developing a technology which enables the decoding of ligand-receptor interactions [1].

Mapping cellular core functions by transposon mutagenesis and next generation sequencing

Paola Picotti was selected as EMBO Young Investigator. Congratulations!

Our US patent 9150916 on “Compositions and methods for identifying the essential genome of an organism” was issued on Oct. 6^th 2015. The Method relates to our PCR based transposon sequencing (TnSeq) strategy to define essential DNA parts of an organism with base pair resolution.

Martin joined the Christen group for his master thesis and Donat started his semester project in our lab. Welcome on board!

In a collaborative effort of the Sauer and Zamboni labs at IMSB and the Berry Lab at NIH, Michael and his co-workers integrated dynamic changes in the secretion and uptake of metabolites with genome-scale metabolic network reconstructions and identified active metabolic routes necessary for mycobacteria survival under hypoxic conditions.

Welcome to Valentina Cappelletti who will visit our lab for several months.